Anti-GFP

Catalog #
AB9066
Family
Fluorescent Proteins
Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Transfected cells
Description
Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
Alternative names
Green fluorescent protein antibody
Immunogen
Purified recombinant peptide produced in E. coli.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in NaHCO3 100 mM pH 9. This antibody is epitope-affinity purified from goat antiserum and does not contain preservatives.
Concentration
2 mg/ml
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:1,000
IHC (P) 1:50-1:1,000
IF 1:50-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ +++ +++ +++ ND +++

320 €

Quantity / Unit Size

400 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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