Anti-mCitrine

Catalog #
AB8262
Family
Fluorescent Proteins
Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mCitrine. mCitrine is a basic (constitutively fluorescent) yellow fluorescent protein engineered from green fluorescent protein (GFP) isolated from Aequorea victoria. It is a ~27 kDa protein that is optimally excited at a 516 nm and has a maximum of emission at 529 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Alternative names
green fluorescent protein, yellow fluorescent antibody.
Immunogen
Purified recombinant fluorescent protein produced in E. coli.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3 mg/ml
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:500
IHC (P) 1:50-1:500
IF 1:50-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ +++ +++ +++ ND

Images

Immunofluorescence – anti-mCitrine Ab using COS7 cells transduced with mCitrine-CAV1; cells were fixed with methanol and anti-mCitrine at 1/250;
Anti-mCitrine Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Immunofluorescence – anti-mCitrine Ab using COS7 cells transduced with mCitrine-CAV1; cells were fixed with methanol and anti-mCitrine at 1/250;

320 €

Quantity / Unit Size

300 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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