Anti-tdTomato

Catalog #
AB7358
Family
Fluorescent Proteins
Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P), IF, ELISA
Reactivity
Transfected cells
Description
Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It is a ~54 kDa protein that is optimally excited at a 554 nm and has a maximum of emission at 581 nm. It´s brightness and emission wavelength, makes it ideal for live animal research.
Alternative names
Cherry fluorescent protein; DsRed, mCherry, red fluorescent protein, RFP antibody.
Immunogen
Purified recombinant peptide produced in E. coli.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in NaHCO3 100 mM pH 9. This antibody is epitope-affinity purified from goat antiserum and does not contain preservatives.
Concentration
2 mg/ml
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 54 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
Special instructions
N/A

Usage

Application Values
WB 1:500-1:2,000
IHC (F) 1:50-1:500
IHC (P) 1:50-1:500
IF 1:50-1:500
ELISA ND

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ +++ +++ +++ ND +++

320 €

Quantity / Unit Size

400 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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