Anti-mCardinal

Catalog #
AB2491
Family
Fluorescent Proteins
Category
Antibodies
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mCardinal (Far Red fluorescent protein). mCardinal is a basic (constitutively fluorescent) far red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity. mCardinal is a ~27 kDa protein that is optimally excited at a 604 nm and has a maximum of emission at 659 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
Alternative names
far-Red antibody.
Immunogen
Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3 mg/ml
Specificity
In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND ND ND

320 €

Quantity / Unit Size

300 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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