Anti-Miranda

Catalog #
AB0344
Family
Insect
Category
Antibodies
Source
Goat
Application
WB, IF
Reactivity
Fruit fly
Description
Goat polyclonal antibody to Miranda. This protein plays a role in neurogenesis in Drosophila embryos.
Alternative names
CG12249, Dmel\CG12249, mir, Mir, MIR, Mira, MIRA antibody.
Immunogen
Recombinant peptide derived from within residues 220 aa to the N-terminus of Drosophila melanogaster Miranda produced in E. coli.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3 mg/ml
Specificity
Detects endogenous levels of Miranda protein by immunofluorescence.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:2,000
IF 1:50-1:500

Reactivity Chart

Sample WB IF
Fruit fly +++ +++

Images

Immunofluorescence – anti-Miranda Ab in fly cells at 1/100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA;
Anti-Miranda Ab at 1/1,000 dilution; protein at 40 ng per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Immunofluorescence – anti-Miranda Ab in fly cells at 1/100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA;

320 €

Quantity / Unit Size

600 µg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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