Anti-ERP57

Catalog #
AB0004
Family
Organelle Markers
Category
Antibodies
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal to ERp57 - endoplasmic reticulum lumen marker. This endoplasmic reticulum protein interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. It has disulfide isomerase activity and complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Alternative names
58 kDa glucose regulated protein, 58 kDa microsomal protein, disulfide isomerase ER 60, endoplasmic reticulum resident protein 57, endoplasmic reticulum resident protein 60, ER protein 57, ER protein 60, ER protein 61, ERP57, ERp60, ERp61, glucose regulated protein 58 Kd, GRP57, GRP58, HsT17083, P58, PDIA3, phospholipase C alpha, PI PLC, protein disulfide isomerase A3 antibody.
Immunogen
Recombinant peptide derived from within residues 300 aa to the C-terminus of human ERp57 produced in E. coli.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Form
Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3 mg/ml
Specificity
Detects a band of 60 kDa by Western blot in the following canine, human, monkey, mouse, rat whole cell lysates.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:2,000
IF 1:50-1:500
IHC (P) 1:200-1:1,000
IHC (F) 1:200-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ +++ ND
Rat +++ +++ +++ +++ ND
Mouse +++ +++ +++ +++ ND
Canine +++ +++ +++ +++ ND
Monkey +++ +++ +++ +++ ND

External References (1)

Images

Immunofluorescence – anti-ERP57 Ab in primary RPE cells at 1/100 dilution; cells were fixed with 4% of PFA;
Endogenous ERP57- endoplasmic reticulum lumen marker detected at 1/500 dilution; lysates at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Immunofluorescence – anti-ERP57 Ab in primary RPE cells at 1/100 dilution; cells were fixed with 4% of PFA;

300 €

Quantity / Unit Size

600 µg
1.5 mg
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Shipping

€30.00 - Portugal (Blue Ice/RT)

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